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. 2004 Oct;24(20):8862–8871. doi: 10.1128/MCB.24.20.8862-8871.2004

FIG. 1.

FIG. 1.

(A) Percent CpG methylation in the indicated ES cell lines as determined by nearest-neighbor analysis. The error bars indicate standard deviations. (B) Percent CpG methylation levels fall in Dnmt[3a−/−,3b−/−] ES cells with continued culture. The relative intensities of the dCp and mdCP spots reflect the frequencies CpG and mCpG at MboI sites. (C) Histone H4 lysine-5 acetylation ([acetyl-K5]H4) levels are increased in Dnmt[3a−/−,3b−/−] P75 ES cells and restored in Dnmt3a and Dnmt3b cDNA-rescued Dnmt[3a−/−,3b−/−] P75 ES cell lines. Acetylation levels are not appreciably increased in Dnmt1−/− ES cells. Separate experiments showing (i) [acetyl-K5]H4 ([ac-K5]H4) levels in Dnmt[3a−/−,3b−/−] P75 ES cells compared with wild-type cells (total H3 serves as a loading control) and (ii) [acetyl-K5]H4 levels in Dnmt1−/− ES cells and the rescued Dnmt[3a−/−,3b−/−] cell lines 3b3 and 3a10 compared with wild-type ES cells. (D) Relative [acetyl-K5]H4 calculated by densitometry after normalization to a total histone H3 loading control.