FIG. 3.

Differentiation is restored by random integration of Dnmt3a and Dnmt3b expression vectors into the Dnmt[3a^−/−,3b^−/−] P75 ES genome. Nearest-neighbor analysis shows the extent of CpG methylation (meth.) in Dnmt3a-rescued (3a4, 3a10, and 3a16) and Dnmt3b-rescued (3b3, 3b17, and 3b23) clones and a mock-rescued clone 1 (A). The percentages of colonies showing erythroid differentiation (diff.) (B) and myeloid differentiation (C) and the percentage of EBs showing cardiomyocyte differentiation (D) are shown. The x axis labels shown in Fig. 3D are the same for all graphs. The error bars indicate standard deviations. ND, not determined. *, occasional beating seen after 15 days.