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. 2004 Oct;24(20):8862–8871. doi: 10.1128/MCB.24.20.8862-8871.2004

FIG. 5.

FIG. 5.

Hypomethylated Dnmt[3a−/−,3b−/−] P75 ES cells fail to initiate differentiation upon LIF withdrawal. ES cells were cultured either with LIF (100 U/ml) or without LIF before being replated at low density into 100 or 0 U of LIF/ml. (A) Alkaline phosphatase staining of colonies previously cultured in LIF (100 U/ml) and replated into 0 U of LIF/ml. (B) Percentages of stem cell (black), mixed (grey), and differentiated (white) colonies observed in panel A. (C) ES cell colonies recovered (100 U of LIF/ml; alkaline phosphatase stain) after growth with and without LIF for 7 days. (D) Total numbers of stem cell (black), mixed (grey), and differentiated (white) colonies recovered after 7 days without LIF. (E) Oct4 and SHP2 Western blot of ES cells grown in 100 or 0 U of LIF/ml for 7 days.