TABLE 1.
S. cerevisiae strains used
| Strain | Descriptiona | Reference |
|---|---|---|
| UTL-7A (wild type) | MATα ura3-52 trp1 leu2-3/112 | 26 |
| UTL-Pex5p-ProtA | UTL-7A PEX5-TEV-protein A-kanMX6-Tpex5 | This study |
| UTL-FOX1-GFP | UTL-7A FOX1-kanMX4-Tfox1 | This study |
| pex5Δ | MATα ura3-52 trp1 leu2-3/112 pex5::LEU2 | This study |
| pex5Δ-Pex5p-N-TEV-ProtA | PEX5(1-313)-TEV-protein A-kanMX6-Tpex5 | This study |
| pex5Δ-FOX1-GFP | pex5::LEU2 FOX1-γEGFP-kanMX4-Tfox1 | This study |
| pex5Δ-FOX1-GFP | pex5::LEU2 FOX1-γEGFP-kanMX4-Tfox1 | This study |
| pex5Δ pex7Δ | pex5::loxP/pex7::LEU2 MATα ura3-52 trp1 leu2-3/112 | This study |
| pex5Δ pex14Δ | pex5::LEU2 pex14::loxP MATα ura3-52 trp1 leu2-3/112 | This study |
| pex5Δ pex14Δ-FOX1-GFP | pex5::LEU2 rpex14::loxP FOX1-γEGFP-kanMX4-Tfox1 | This study |
| pex5Δ pex12Δ | pex5::LEU2 pex12::loxP MATα ura3-52 trp1 leu2-3/112 | This study |
| pex5Δ pex12Δ-FOX1-GFP | pex5::LEU2 pex12::loxP FOX1-γEGFP-kanMX4-Tfox1 | This study |
| pex5Δ pex8Δ | pex5::LEU2 pex8::loxP MATα ura3-52 trp1 leu2-3/112 | This study |
| pex5Δ pex8Δ-FOX1-GFP | pex5::LEU2 pex8::loxP FOX1-γEGFP-kanMX4-Tfox1 | This study |
a
Additional proteins expressed from plasmids are indicated by parentheses.