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The protein kinase inhibitor H8 specifically inhibits pre-mRNA processing coupled to transcription. (A) RPA of β-globin intron 1 splicing from oocytes injected with CMV β-globin SPA and treated with 0, 25, 50, or 100 μg of H8/ml (lanes 1 to 4). RPA of capped β-globin pre-mRNA injected into oocyte treated with 0 or 200 μg of H8/ml (lanes 5 and 6). (B) RPA of SPA site cleavage with the same RNA samples as those used for the experiment depicted in panel A.
