FIG. 3.

Morphology and ultrastructure of the iridocorneal angle. Representative H&E-stained sections of eyes from a 25-month-old control (wild type [WT]) mouse (A) and a 27-month-old Tg/Tg mouse (B). The iridocorneal angle, TM (arrowhead), and Schlemm's canal (between arrows) are not affected by genotype and appear normal. The eyes of mice of both genotypes contain pigment-filled cells (asterisks). This is typical in C57BL/6J mice at this very old age and is not a phenotypic consequence of the transgene. There were no detectable histological differences in the iridocorneal angles of each genotype between 18 and 30 months of age. Scale bar, 40 μm. Electron micrographs of eyes from 20-month-old control (C) and Tg/Tg (D) mice. Similar results were observed in mice more than 2 years old. The TM of mice of each genotype has a normal morphology including an endothelium-lined Schlemm's canal (SC), trabecular beams with organized collagen fibrils (filled arrowhead) and elastic tissue (open arrowhead), and open intertrabecular spaces (arrow). The anterior chamber is identified as AC. A normal myelinated nerve (N) is visible in the transgenic eye. Scale bars, 1 μm.