FIG. 6.
Effect of Eregtm1Dwt on response to DSS treatment. (a) Adult male mice (n = 10 for wild-type and n = 9 for Ereg null mice) were fed 1.5% (wt/vol) DSS in drinking water. Body weight was measured at the indicated time after treatment and expressed as a percentage of the weight on the day of first exposure to DSS. Solid circles, wild-type; open circles, Ereg null mice. Data are means ± standard deviation. *, P < 0.05; and **, P < 0.005, based on a Mann-Whitney test. The colons were collected 9 days after treatment was initiated. (b) Blood content was determined by measuring the portion of the area including blood from the entire colon. (c) Histological damage was scored from cross sections of the colon as described in Materials and Methods. Each dot represents an individual mouse of the wild-type (+/+, solid circles) and Ereg null (−/−, open circles) mice. Blood content and histological score were significantly different (P < 0.0001 with an unpaired t test) between wild-type and Ereg null mice. The colons of Ereg null mice were collected either after no treatment (d to f) or after 9 days of DSS treatment (g to i) and stained with X-Gal. (d and g) Proximal colon; (e and h) mid-colon; (f and i) proximal colon. Arrows numbered 1 to 3 in h indicate histological damage scores as described in Materials and Methods.