FIG. 1.

Representatives of group A and group E, two sets of differentially regulated dE2F target genes. (A) Western blot analysis of whole-cell extracts from dE2F RNAi-treated cells. Cells were incubated with dsRNA targeting dE2F1, dE2F2, or dDP for 8 days. A luciferase control was used as a nonspecific (NS) double-stranded RNA. Each blot was probed with two antibodies, one specific for the RNAi target protein and tubulin as a loading control. Note that three different forms of dE2F2 are recognized by multiple monoclonal and polyclonal antibodies to dE2F2. The reason for the varied mobility of dE2F2 is not known. (B) Same as panel A except cells were incubated with dsRNA targeting RBF1, RBF2, or both RBFs (RBF1/RBF2). (C) Northern blot analysis of total RNA extracted from RNAi-mediated depleted cells. Blots were probed for the expression of A genes (DNK and RNR2), E genes (ARP53D and CG17142), and RP49 (control).