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DNA binding by p53 protein after UV irradiation. EMSAs were performed using a p53-specific oligonucleotide probe, in wild-type (+/+), p53S389A/S389A (SA/SA), and p53−/− (−/−) MEFs. All samples were isolated 6 h after irradiation with 20-J/m2 UV-C and were incubated with the labeled probe in the presence or absence of p53-specific antibody and wild-type (WT) cold competitor.
