Skip to main content
. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biopolymers. 2016 Mar;105(3):143–162. doi: 10.1002/bip.22762

FIGURE 12.

FIGURE 12

Structural analysis of multi-phosphorylated TM4-Cx43CT domains. A) CD and 15N-HSQC spectra of the (B) TM4-Cx43CT assembly(S325,328,330,365D) and (C) disassembly(Y247,265;S255,262,279,282,368D) phosphomimetic constructs in 10% TFE, MES buffer, 8% LPPG, and at pH 5.8. Black circles highlight the glycine residues. From the 15N-HSQC data, each (D) TM4-Cx43CT assembly(S325,328,330,365D) and (E) disassembly(Y247,265;S255,262,279,282,368D) residue was plotted against their change in chemical shift (Δσ =√((ΔδHN)2 + (ΔδN/5))2) as compared to TM4-Cx43CT wild-type. The asterisks denote the serine/tyrosine residues substituted for an aspartic acid.