Table III.

Mean Residue Ellipticity (MRE) Values at pH 7.5 for TM4-CxCT Domains

Isoform	TFE (%)	First local minimum		Minimum at 222 nm	Totala/CT-only
		Wavelength (nm)	MRE	MRE	α-helical content (%/%)
TM4-Cx26CT	0	208.8	−30,335.5	−26,678.9	60.0/13.7 (1.2)b
	5	208.9	−31,188.1	−26,874.9	60.9/14.6
	10	208.7	−31,160.2	−26,903.5	57.8/11.5
	15	209.4	−32,470.1	−29,004.2	56.7/10.4
	30	210.2	−31,068	−32,315.6	64.4/18.1 (1.6)
TM4-Cx32CT	0	207.8	−11,847.7	−9582.89	29.7/4.5 (3.0)
	5	207.8	−12,835.4	−10,243.6	31.1/5.9
	10	208.5	−13,073	−10,642.7	32.0/6.8
	15	208.2	−13,565.2	−11,151.4	32.0/6.8
	30	208.8	−13,995.8	−12,496.3	35.1/9.9 (6.6)
TM4-Cx37CT	0	208	−8901.92	−6454.69	18.4/0.0 (0.0)
	5	207.5	−10,017.3	−7408.12	18.1/0.0
	10	207.9	−9416.02	−7260.21	18.0/0.0
	15	207.8	−10,269.5	−7827.59	19.0/1.0
	30	208	−8591.11	−6939.2	18.2/0.0 (0.0)
TM4-Cx40CT	0	207.8	−4007.67	−3182.69	12.0/0.0 (0.0)
	5	208	−4434.49	−3549.43	14.1/0.0
	10	207.9	−4540.67	−3643.55	14.4/0.0
	15	208.5	−4777.48	−3958.42	14.5/0.0
	30	208.8	−4830.4	−4318.5	14.1/0.0 (0.0)
TM4-Cx43CT	0	207.2	−7856.02	−5348.97	17.4/2.9 (4.3)
	5	207.2	−8010.42	−5721.19	20.0/5.5
	10	207.3	−8269.2	−5900.64	22.0/7.5
	15	207.6	−8644.65	−6253.59	22.7/8.2
	30	207.5	−8084.19	−6271.77	21.1/6.6 (9.8)
TM4-Cx45CT	0	208.3	−9245.19	−7552.05	24.1/8.6 (12.0)
	5	208.2	−9998.6	−8072.77	25.6/10.1
	10	208.4	−11,166.2	−9102.24	28.0/12.5
	15	208.8	−11,242.3	−9443.91	29.4/13.9
	30	208.6	−12,066.6	−10,425.9	30.7/15.2 (21.3)
TM4-Cx50CT	0	206.3	−3385.69	−2481.02	10.1/0.0 (0.0)
	5	205.9	−3178.23	−2208.79	10.4/0.0
	10	207.6	−3655.45	−2583.12	11.7/0.2
	15	207.3	−4103.86	−2983.17	11.3/0.0
	30	207.9	−4148.69	−3287.84	12.8/1.3 (2.6)

^aDetermined using DichroWeb.

^bEstimate of the total number of CT residues structured as an α-helix. The contribution of the TM4 α-helix was subtracted from the total α-helix percentage, leaving the remaining α-helical content from the CT domain.