Table IV.

Mean Residue Ellipticity (MRE) Values for TM4-Cx32 Mutants

Mutant	TFE (%)	First local minimum		Minimum at 222 nm	Mutant/wild-type
		Wavelength (nm)	MRE	MRE	α-helical contenta (%/%)
R219H (pH 7.5)	0	207.8	−12,530.0	−9927.17	32.2/29.7
	5	207.7	−12,568.5	−10,142.5	32.7/31.1
	10	208.1	−13,948.0	−10,517.5	34.6/32.0
	15	207.5	−13,406.6	−10,609.2	33.1/32.0
	30	208.1	−14,313.3	−11,706.5	34.8/35.1
F235C	0	207.6	−12,452.2	−10,002.6	27.2/29.7
	5	208.3	−11,036.3	−8606.9	27.9/31.1
	10	207.7	−12,067.0	−9631.32	30.0/32.0
	15	207.9	−12,919.7	−10,171.6	30.7/32.0
	30	208.3	−13,264.3	−12,797.9	35.2/35.1
R230C	0	208.1	−8742.33	−6642.65	22.6/29.7
	5	207.3	−11,553.6	−9285.26	29.1/31.1
	10	208.7	−11,627.6	−9443.29	28.1/32.0
	15	207.4	−11,847.4	−9827.88	29.1/32.0
	30	208.6	−11,266.6	−11,369.7	29.0/35.1
R219H (pH 5.8)	0	207.8	−13,036.5	−10,106.4	33.1/36.9
	5	206.7	−14,228.3	−10,799.7	35.0/35.8
	10	207.8	−15,595.8	−12,202.2	38.0/35.3
	15	208.4	−14,373.3	−11,403.0	34.1/36.9
	30	208.9	−15,200.0	−13,093.8	37.7/37.8
F235C	0	207.6	−12,410.2	−9634.2	31.1/36.9
	5	208.0	−13,024.0	−10,391.2	31.6/35.8
	10	207.5	−13,102.5	−10,448.6	31.6/35.3
	15	207.8	−14,428.5	−11,428.0	34.4/36.9
	30	208.5	−16,281.5	−13,789.1	37.8/37.8
R230C	0	208.0	−12,934.5	−10,554.6	34.0/36.9
	5	208.0	−13,246.9	−10,711.8	32.6/35.8
	10	208.0	−13,315.1	−10,649.4	33.2/35.3
	15	208.1	−12,606.1	−10,457.6	31.3/36.9
	30	208.0	−15,756.6	−13,806.9	38.6/37.8

^aDetermined using DichroWeb.