Table V.

Mean Residue Ellipticity (MRE) Values for TM4-Cx26 Mutants

Mutant	TFE (%)	First local minimum		Minimum at 222 nm	Mutant/wild-type
		Wavelength (nm)	MRE	MRE	α-helical contenta (%/%)
L214P (pH 7.5)	0	210.3	−18,844.7	−17,872.6	44.2/60.0
	5	210.5	−27,498.3	−26,284.4	62.7/60.9
	10	210.4	−27,512.8	−26,372.5	63.0/57.8
	15	210.4	−30,011.3	−28,779.1	64.9/56.7
	30	210.0	−23,204.7	−26,477.1	61.3/64.4
K224Q	0	208.1	−18,367.3	−16,613.3	45.3/60.0
	5	208.4	−23,213.1	−20,380.8	57.2/60.9
	10	208.6	−20,160.5	−17,594.9	59.1/57.8
	15	209.8	−24,329.1	−21,359.5	56.4/56.7
	30	209.9	−29,079.6	−29,287.3	67.7/64.4
L214P (pH 5.8)	0	210.1	−23,122.6	−21,853.3	55.6/64.9
	5	209.4	−30,183.7	−29,088.5	68.4/67.1
	10	210.4	−32,918.3	−30,952.7	69.1/66.3
	15	212.1	−35,867.7	−33,071.2	70.9/68.9
	30	212.9	−30,496.6	−32,621.8	62.2/70.8
K224Q	0	208.0	−24,502.0	−22,203.1	61.4/64.9
	5	208.7	−25,288.7	−22,080.1	59.2/67.1
	10	208.3	−26,554.7	−23,673.6	62.1/66.3
	15	208.6	−27,384.7	−24,630.1	63.8/68.9
	30	210.0	−32,707.8	−30,550.2	70.9/70.8

^aDetermined using DichroWeb.