Fig 1.

Activity test of recombinant human CPR (A) and recombinant human CYP3A4, CYP2B6, and CYP2D6 (B). 3A4+, 2B6+, and 2D6+ are microsomes of clones coexpressing both CPR and CYP genes; 3A4–, 2B6–, and 2D6– or CPR–are microsomes of clones expressing only one gene, the CYPs or CPR, respectively; NC (negative control) are microsomes of clones bearing the control pESC-URA plasmid. The standard deviation values of the measurements of fluorescence microplate assay (B) were all less than 5% of the calculated values and are thus not presented here.