Table 2. Transcripts of interest from the cluster analysis of significant Depot*[Dex] interactions (Fig 2).
| CLUSTER | PATTERN | GENES OF INTEREST | B IOLOGICAL PROCESS IMPLICATED |
|---|---|---|---|
| 1 | Similar baseline; Dex upregulated, greater sensitivity and response in Abdsc | CYP4B1, CYP4F22, CYP4X1, CYP4Z1 | FA, steroid, lipid, xenobiotic metabolism [25] |
| 2 | Lower baseline in Om; Dex upregulated, lower sensitivity and greater response in Om | CD10 (MME) | Adipose stem cell marker [26] |
| ENPP2 (autotaxin) | Anti-adipogenic [27] | ||
| 3 | Similar baseline; Dex upregulated, greater sensitivity in Abdsc | LEP | Adipokine |
| 4 | Similar baseline; Dex upregulated in Om, no response in Abdsc | MUC16 | ECM, PM [28] |
| MMRN1 | PM adhesion/ coagulation [29] | ||
| ITLN1 | Inflammation/adipokine [30] | ||
| ACSM3, AGPAT9 | TAG synthesis [31] | ||
| 5 | Similar or higher baseline in Abdsc; Dex upregulated in Om, downregulated in Abdsc | FADS1 | Delta 5 fatty acid desaturation |
| GPC4 | Proadipogenic; ↑insulin signaling; ECM, PM [32] | ||
| 6 | Higher baseline in Om; Dex downregulated in Om, no effects in Abdsc | PI15 | ECM, peptidase inhibitor |
| TNFRSF21, LEPR | ECM, cytokine-related | ||
| DDIT4 | Inhibits TORC1 signaling [33] | ||
| THBS2 | ECM-receptor interaction, focal adhesion; potential role in adipogenesis [34] | ||
| 7 | Similar baseline; Dex downregulated, greater sensitivity and response in Abdsc | LTBP1 | ECM, inhibits TGFβ signaling |
| BGN | ↑ in obesity (BGN), adipose inflammation [35] | ||
| THBS1 | Regulates adipose expansion [36] | ||
| 8 | Higher baseline in Om; Dex downregulated in Om, lower or little response in Absc | WNT4FLT1 | Pro-adipogenic [37]PM, pro-angiogenic; improved insulin action [38] |
| GPR116 | Cell adhesion, promotes insulin sensitivity [39] | ||
| INHBB | Decrease lipolysis [40] | ||
| LOXL2 | Fibrosis [41] | ||
| 9 | Similar baseline; 1 nM Dex slightly downregulated in Om, upregulated in Abdsc | NRN1 | Expressed in human adipose progenitors [26] |
| HOXC8, HOXC9 | Developmental/higher in Abdsc (fresh tissue) [42] | ||
| DKK2 | Inhibits WNT signaling (proadipogenic) | ||
| 10 | Higher baseline in Abdsc; Dex downregulated in Abdsc, lower response in Om | GREM2 | Stimulates Wnt signaling (anti-adipogenic) [43] |
| CCL13 | Inflammation/increased in obesity[44] | ||
| DDIT1L | Inhibits cell growth, TOR signaling pathway [45] |
Transcripts were selected for inclusion in this Table if they exhibited a consistent ~2-fold change in one depot and the literature suggests that they may play a role in mediating depot differences in fat accumulation or depot-dependent function. Potential biological pathways/processes that may be modulated by each gene product were based on our review of the literature (Pubmed searches on the gene name and the search terms “adipose OR adipocyte”) and/or information in www.genecards.org. The complete lists of genes are in S3 Table. Plasma membrane (PM) or ECM localization of gene products is noted.