Fig 4. Characterization of the jujube S locus.

(A) Phylogenetic analysis of three T2-RNase sequences from each ‘Junzao’ (Zj.jz) and ‘Dongzao’ (Zj.dz) with other known S genes. (B) Expression (RPKM) of S-RNase and S-like RNase genes in different tissues. (C) Physical positions of S-RNase and SFB genes on chromosome 1 of the ‘Junzao’ genome. The scaled log₂ expression values (RPKM) are shown in the heat map. From left to right, leaf (L), flower (F), phloem (P), expanding fruit (EF), half-red fruit (HR) and full-red fruit (FR). (D) Frequency of S-locus genotypes in 32 jujube accessions.