Fig 2. Interspecies specificity of the antibodies used for immunohistochemistry.

(A) Western blot analysis of EGFR, USP8 and α-tubulin expression proteins extracted from dog testis, dog pituitary adenoma and normal pituitary gland and from the human adrenocortical cell-line NCI-H295. (B) Protein sequence conservation status between human (upper sequence) and dog (lower sequence) in the area of the anti human USP8 antibody epitope (amino-acids 239–377, blue colour).