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. 2016 Dec 22;11(12):e0168442. doi: 10.1371/journal.pone.0168442

Table 1. Synergistic effects between Cry1Ac and Cry1Ie toxins on ACB.

ACB-strain Toxins proportions (Cry1Ac:Cry1Ie) LC50 (95%FL) (μg/g) Expected LC50 (μg/g) SF χ2
ACB-BtS 0.17:0.83 6.13 (4.20–8.34) 13.93 2.3 66.747
0.33:0.67 2.85 (1.92–3.79) 8.83 3.1 42.951
0.50:0.50 1.43 (0.97–1.96) 6.46 4.5 47.116
0.67:0.33 2.28 (1.88–2.71) 5.09 2.2 36.012
0.83:0.17 0.80 (0.65–0.97) 4.21 5.3 40.859
1:0 3.58 (3.11–4.08) 24.247
0:1 32.97 (24.03–43.43) 47.467
ACB-AcR 0.17:0.83 86.27 (72.52–100.05) 59.88 0.6N 14.457
0.33:0.67 63.73 (52.32–75.38) 73.53 1.2N 23.621
0.50:0.50 74.90 (52.07–100.00) 96.15 1.3N 48.133
0.67:0.33 32.74 (23.09–42.20) 136.99 4.2 28.667
0.83:0.17 42.50 (34.39–50.36) 243.90 5.7 8.529
1:0 >1000
0:1 50.2 9(40.47–61.08) 41.664
ACB-IeR 0.17:0.83 19.09 (15.530–23.11) 37.59 2.0 34.692
0.33:0.67 5.47 (4.15–7.06) 20.83 3.8 49.292
0.50:0.50 5.83 (4.27–7.62) 14.29 2.5 53.200
0.67:0.33 5.36 (3.09–7.68) 10.99 2.1 47.706
0.83:0.17 3.24 (2.37–4.31) 8.85 2.7 42.408
1:0 7.43 (6.15–8.71) 22.438
0:1 >200
ACB-FR 0.17:0.83 25.55 (21.81–29.53) 42.92 1.7 29.077
0.33:0.67 21.48 (18.70–24.35) 32.36 1.5 12.321
0.50:0.50 14.19 (11.43–17.16) 25.97 1.8 38.739
0.67:0.33 4.83 (4.00–5.76) 21.74 4.5 45.239
0.83:0.17 9.14 (7.92–10.43) 18.66 2.0 15.086
1:0 16.35 (12.35–20.42) 42.338
0:1 63.52 (49.34–78.29) 43.428

SF: synergism factor;

N: no synergism effect