Table 2. Mortality of ACB larvae of four strains fed on plant tissues of Bt corn and non-Bt corn hybrids.
| Insect strains | Corn hybrids | Mortalities % | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Whorl | Tassel | Silk | Husk | ||||||||||
| 2 d | 4 d | 6 d | 2 d | 4 d | 6 d | 2 d | 4 d | 6 d | 2 d | 4 d | 6 d | ||
| ACB-BtS | Zh58Z31 | 6.9±1.4 c | 7.6±1.3 b | 11.8±1.3 b | 13.9±1.4 b | 10.8±2.0 c | 15.8±2.7 c | 27.5±3.6 c | 9.2±2.0 c | 15.8±3.3 b | 19.2±3.3 b | ||
| Zh58Ie | 99.3±0.7 a | 100.0±0.0 a | 98.6±1.0 a | 100.0±0.0 a | 64.6±2.7 b | 69.2±3.1 b | 78.8±1.1 b | 95.8±1.4 b | 99.2±0.5 a | 99.6±0.4 a | |||
| Z31Ac | 95.1±2.0 b | 97.9±1.4 a | 99.3±0.7 a | 100.0±0.0 a | 85.0±5.32 a | 93.3±4.9 a | 95.8±3.3 a | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a | |||
| AcIe | 96.5±1.3 ab | 99.3±0.7 a | 100.0±0.0 a | 100.0±0.0 a | 64.2±6.0 b | 80.0±2.9 b | 82.5±2.5 b | 96.7±3.3 ab | 98.3±1.7 a | 99.2±0.8 a | |||
| F3, 20 | 176.55 | 339.6 | 402.5 | 3220.74 | 39.92 | 50.75 | 61.97 | 142.42 | 207.79 | 277.11 | |||
| P | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | |||
| ACB-AcR | Zh58Z31 | 18.1±1.4 c | 27.8±1.8 c | 31.3±1.4 c | 13.9±1.8 c | 17.4±2.0 c | 21.5±3.3 c | 5.8±3.3 c | 10.0±3.7 c | 21.7±4.8 b | 5.8±1.5 d | 10.8±2.4 d | 16.7±2.8 d |
| Zh58Ie | 98.6±0.7 a | 100.0±0.0 a | 100.0±0.0 a | 97.2±1.0 a | 97.6±1.1 a | 98.6±0.7 a | 32.1±3.3 a | 42.1±4.3 a | 54.2±3.4 a | 76.7±4.1 b | 91.7±1.4 b | 94.2±1.4 b | |
| Z31Ac | 36.1±3.3 b | 52.1±2.8 b | 62.5±1.9 b | 45.8±2.9 b | 54.9±2.0 b | 60.4±3.2 b | 16.7±2.1 b | 19.2±2.4 b | 38.3±2.1 a | 33.3±5.6 c | 40.0±7.3 c | 50.0±8.4 c | |
| AcIe | 99.3±0.7 a | 100.0±0.0 a | 100.0±0.0 a | 95.8±1.5 a | 96.5±1.7 a | 97.9±1.4 a | 18.3±3.8 b | 23.3±5.1 b | 53.3±6.2 a | 96.7±2.1 a | 98.3±1.1 a | 100.0±0.0 a | |
| F3, 20 | 339.60 | 923.53 | 1575.35 | 165.55 | 139.27 | 138.52 | 10.81 | 9.19 | 9.07 | 91.03 | 112.65 | 111.9 | |
| P | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | 0.0002 | 0.0005 | 0.0005 | <.0001 | <.0001 | <.0001 | |
| ACB-IeR | Zh58Z31 | 6.9±1.4 d | 13.9±2.3 c | 16.7±1.9 c | 6.3±1.8 c | 13.2±2.0 c | 17.4±2.3 c | 3.3±1.7 c | 13.3±1.1 b | 20.0±1.84 c | 5.8±1.5 c | 13.3±1.1 c | 20.8±1.5 c |
| Zh58Ie | 22.9±3.1 c | 26.7±2.7 b | 36.5±4.4 b | 46.5±1.3 b | 51.7±1.3 b | 55.6±1.0 b | 10.8±1.9 b | 20.8±1.7 b | 27.5±2.7 b | 33.3±2.5 b | 38.3±5.2 b | 49.6±4.7 b | |
| Z31Ac | 95.8±1.1 b | 96.5±1.3 a | 98.6±0.9 a | 97.2±1.4 a | 97.2±1.9 a | 99.3±0.7 a | 20.0±3.2 b | 31.7±3.8 a | 47.5±2.5 a | 97.5±1.7 a | 99.2±0.8 a | 100.0±0.0 a | |
| AcIe | 99.3±0.7 a | 100.0±0.0 a | 100.0±0.0 a | 97.2±1.4 a | 99.3±0.7 a | 100.0±0.0 a | 31.7±3.8 a | 36.7±4.4 a | 42.5±4.0 a | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a | |
| F3, 20 | 337.11 | 432.20 | 293.11 | 135.80 | 242.24 | 573.49 | 19.09 | 12.07 | 269.41 | 256.45 | 323.36 | 478.90 | |
| P | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | |
| ACB-FR | Zh58Z31 | 10.4±1.4 d | 14.6±1.8 c | 19.4±2.3 c | 9.7±1.8 c | 18.1±1.4 b | 8.3±1.1 c | 17.5±2.4 c | 31.7±3.1 bc | 11.7±1.1 d | 19.2±3.0 c | 32.5±2.8 c | |
| Zh58Ie | 98.3±1.0 b | 100.0±0.0 a | 100.0±0.0 a | 98.3±0.8 ab | 100.0±0.0 a | 31.3±2.8 a | 33.8±3.3 ab | 42.1±2.3 b | 91.9±2.5 b | 96.7±2.0 a | 100.0±0.0 a | ||
| Z31Ac | 78.5±2.3 c | 90.3±1.8 b | 96.5±1.3 b | 95.8±1.9 b | 100.0±0.0 a | 20.8±4.2 b | 23.3±5.4 bc | 27.5±5.9 c | 60.4±5.7 c | 66.7±4.9 b | 76.7±4.6 b | ||
| AcIe | 100.0±0.0 a | 100.0±0.0 a | 100.0±0.0 a | 99.3±0.7 a | 100.0±0.0 a | 40.8±4.7 a | 44.2±5.1 a | 62.5±3.1 a | 99.2±0.8 a | 100.0±0.0 a | 100.0±0.0 a | ||
| F3, 20 | 308.18 | 804.1 | 348.1 | 191.43 | 3717.97 | 17.47 | 7.52 | 13.68 | 123.28 | 130.31 | 211.86 | ||
| P | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | 0.0015 | 0.0002 | <.0001 | <.0001 | <.0001 | ||
The mean±SE in the same column followed by the same letter were not significantly different by Fisher’s Protect LSD test (P ≥ 0.05).
The Zh58Z31 corn line is a hybrid of the non-transgenic counterpart lines Zong 31 (Z31) and Zheng 58 (Zh58) was used as a negative control. The Zh58Ie corn line is a hybrid that only expresses Cry1Ie. The Z31Ac corn line is a hybrids that only expresses Cry1Ac. The AcIe corn strain, is a hybrid that expresses both Cry1Ac and Cry1Ie.