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. 2016 Dec 22;11(12):e0168229. doi: 10.1371/journal.pone.0168229

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© 2016 Komina et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Flow cytometry showed a significant increase in apoptotic BRO melanoma cells after miR-4286 inhibition. (B) The percentage of viable, early apoptotic, late apoptotic/necrotic BRO cells. (C) Fluorescent microscopy of alive and apoptotic BRO cells: viable cell nuclei are colored blue, and the cytoplasm of apoptotic caspase-3⁺/7⁺ cells is colored green. *—significant difference compared to the apoptosis rate of negative control cells. (D) Flow cytometry revealed no changes in apoptosis of SK-MEL-1 cells after miR-4286 inhibition. (E) The percentage of viable, early apoptotic, late apoptotic/necrotic SK-MEL-1 cells. (F) Fluorescent microscopy of alive and apoptotic SK-MEL-1 cells: viable cell nuclei are colored blue, and the cytoplasm of caspase-3⁺/7⁺ apoptotic cells is colored green.