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. 2016 Dec 12;10(12):e0005225. doi: 10.1371/journal.pntd.0005225

Fig 4. Setting up LAMP assay.

Fig 4

LAMP amplification results using (A) 1 ng, (B) 1 pg and (C) 1 fg of S. mansoni DNA obtained at different incubation times (10, 20, 30, 40, 50 and 60 min) tested in a heating block by the addition of SYBR Green I (top) or by visualization on agarose gel (bottom). Lanes M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lanes N: negative control (no DNA template). (D) Sensitivity assessment performed with LAMP at 63°C for 50 min using serial dilutions of S. mansoni genomic DNA. Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche); lane Sm: genomic DNA from S. mansoni (1 ng); lanes 10−1–10−9: 10-fold serially dilutions; lane N: negative control (no DNA template). (E) Specificity of the LAMP assay for S. mansoni. A ladder of multiple bands of different sizes could be only observed in S. mansoni DNA sample. Lane M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lanes Sm, Sh, Sj, Si, Sb, Fh and Dd, S. mansoni, S. haematobium, S. japonicum, S. intercalatum, S. bovis, Fasciola hepatica and Dicrocoelium dendriticum DNA samples (1 ng/each), respectively; lane N, negative control (no DNA template).