Fig 1. Kinetics for clearance of CPS from serum.
Mice were intravenously injected with 100 μg, 20 μg, or 4 μg of CPS. Blood samples were collected at the designated time points, and CPS concentrations in serum samples were determined using quantitative sandwich ELISA. The data were best described by a two-parameter monophasic exponential decay model (y = ae-bx), where a is the Y intercept and b is the rate constant for clearance. Data shown are mean ± standard deviation for five mice per dose per time point. Half-life (t1/2) values calculated from the elimination rate constant (b) derived from the model fitting were similar for all three doses of CPS. The results demonstrate that CPS is eliminated rapidly from serum with a half-life of 4 hours, 4.4 hours, or 2.9 hours for the doses of 100 μg, 20 μg, or 4 μg CPS, respectively.