Transmission electron micrographs of E. longa strain JUm408 after high-pressure freezing/freeze substitution. A.
E. longa meront. A nucleus is visible in the cytoplasm full of ribosomes. B. Lower magnification with a multinucleated meront. Two meronts are indicated, one with a single nucleus in the plane of section (left) and one with several nuclei (right). Two host nuclei are visible on the right, with a dark nucleolus. Intestinal cells contain two nuclei. C. Early sporonts with an electron-dense coat indicated by arrowhead. D. Sporont undergoing a cell division (big arrow); small arrow indicates junction of host intestinal cells; the arrowhead indicates a host Golgi apparatus. E. Mitotic spindle (arrows designate microtubules) in a sporont; the spindle plaque is indicated by an arrowhead. F. Nascent polar tube (arrow) in a sporoblast. G. Wrinkled sporoblasts (*). Arrows indicate the host rough endoplastic reticulum folding around the microsporidia. H. Late stage sporoblast in the center, mature spore on the top left; arrows indicate polar tubes. I. Mature spore with the anterior part of the polar tube, including the anchoring disk. J. Cross-section of mature spores. The exospore and endospore layers are shown in the inset. Arrowheads indicate polar tubes. K. Two mature spores in the intestinal lumen that do not show an additional membrane around them. Low magnification inset shows the positions of the two spores in the lumen and arrowhead indicates host microvilli. L. Low magnification view of cross-section of host, with the intestinal lumen in the center. E. longa spores (arrowheads) concentrate around the apical membrane of intestinal cell, while meronts and early sporonts are on the basal side. Scale bar is 500 nm, unless indicated otherwise. A, anchoring disk; Chr: chromatin; Ex, exospore; En, endospore; Lu, lumen; M, meront; Mi: host mitochondrion; Mv, host microvilli; Nu, nucleus; HNu host nucleus; Pt, polar tube; RER, rough endoplastic reticulum; St: sporont.