Table II.
Primer sequences used in this study.
| Primer name | Primer sequences (5′→3′) |
|---|---|
| U6-OF | CTCGCTTCGGCAGCACA |
| U6-OR | AACGCTTCACGAATTTGCGT |
| Klf4-OF | ACCCTGGGTCTTGAGGAAGT |
| Klf4-OR | CATGAGCTCTTGGTAATGGAGC |
| Klf4-siKlf4 | AUCGUUGAACUCCUCGGUCUCUCUC |
| Pri-miR-31-OF | GAACTACCCACAAACCTCCTG |
| Pri-miR-31-OR | GGTGTGTCCAAGGAATAGCCA |
| miR-31-OF | TAATACTGCCTGGTAATGATGA |
| miRNA-OR | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCTAT |
| miR-31-promoter(−1069To+1)-F(MluI) | CCGGACGCGTGCACAAAAGTTATACATAATGTCATTATTCTTATG |
| miR-31-promoter(−1069To+1)-R(HindIII) | GCCCAAGCTTCAGTTCCAAGTTACAGGAGAATACTATGA |
| Klf4-miR-31-mut-F1 | AATTAATGAGTGTGTTTACGCACGCACAGGTGAAAGGAAAAA |
| Klf4-miR-31-mut-R1 | TTTTTCCTTTCACCTGTGCGTGCGTAAACACACTCATTAATT |
| Klf4-miR-31-mut-F2 | AGTTGCTTGAGAGGCTACGCTCGCACGATTCACCTGAGCCTG |
| Klf4-miR-31-mut-R2 | CAGGCTCAGGTGAATCGTGCGAGCGTAGCCTCTCAAGCAACT |
| Klf4-miR-31-mut-F3 | GAGCATTATTTTTGGCACGCCTTCGCGGCTCATGCCTGTAAT |
| Klf4-miR-31-mut-R3 | ATTACAGGCATGAGCCGCGAAGGCGTGCCAAAAATAATGCTC |
| Negative control sense | UUCUCCGAACGUGUCACGUTT |
| Negative control antisense | ACGUGACACGUUCGGAGAATT |
| Hsa-miR-31 mimics sense | AGGCAAGAUGCUGGCAUAGCU |
| Hsa-miR-31 mimics antisense | CUAUGCCAGCAUCUUGCCUUU |
| MircoRNA inhibitor NC | CAGUACUUUUGUGUAGUACAA |
| Hsa-miR-31 inhibitors | AGCUAUGCCAGCAUCUUGCCU |
Mutant sequences are underlined.