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. 2016 Nov 16;39(1):135–143. doi: 10.3892/ijmm.2016.2803

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Copyright: © Ning et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Osteogenic marker expression at the protein level. (A) Western blot analysis of thoracic ossification of ligament flavum (TOLF) and non-TOLF samples. (B and E) Semi-quantitative analysis of protein expression in non-TOLF and single- and multiple-level TOLF. (B) Alkaline phosphatase (ALP), (C) osterix, (D) bone morphogenetic protein-2 (BMP2), and (E) osteopontin (OPN) (mean ± standard deviation; n=3 patients/group). ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. non-TOLF at the same time point. ^#P<0.05, ^##P<0.01 vs. single-level TOLF at the same time point.