Figure 1.

Effect of Humulus japonicus (HJ) on lipopolysaccharide (LPS)- induced nitric oxide (NO) production by BV2 microglial cells. BV2 microglial cells were co-treated with 100 and 500 µg/ml concentrations of HJ with LPS (1 µg/ml). Following incubation for 24 h, (A) NO production and (B) the mRNA level of inducible nitric oxide synthase (iNOS) were measured using the culture supernatants of BV2 cells and their lysates, respectively. ^*p<0.05, and ^**p<0.01, significant differences from the LPS-treated group; ^##p<0.01, significant differences from the control group (CTL), as shown by the Student's t-test. Data are presented as the means ± SEM.