Fig 2. System development.

A) Endosomal tubulation in mock transfected and spastin depleted cells. In mock transfected cells (left panel) the endosomal resident protein SNX1 marks an endosomal subdomain and short endosomal tubules. These elongate in cells treated with spastin siRNA (right panel). Scale bar = 10μm. B) Results of automated tubule analysis with data used to develop the system. Increases in endosomal tubulation are observed in all three analysis types. The same data, manually counted, is shown for comparison. True mean length of longest tubule per image can be calculated by adding 2μm to the values shown in the length parameter histogram. 30 images were taken per condition, n = 1.