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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Biopolymers. 2017 Mar;107(3):10.1002/bip.23001. doi: 10.1002/bip.23001

Figure 1.

Figure 1

Effect of the extended peptide linker on the functional properties of DbpA. (A) The RNA construct used for these studies. (B) Binding of wild-type and extended DbpA to 32-mer RNA (construct A) as measured by gel shift assay. (C) ATPase activity of wild-type (circles) and extended DbpA (squares) as measured by coupled NADH/phosphoenolpyruvate assay. (D) Helicase activity of wild-type (circles) and extended DbpA (square). The data are representative of one experiment. The averages of multiple experiments and the standard deviations are shown in Table 1 and Table 2. Representative gel for the binding and the helicase assays are shown in the Supplemental Materials.