Table 2.
Helicase Parameters of wild-type and extended DbpA interacting with various RNA molecules.
| DbpA constructs | RNA | kobsa(min−1) |
|---|---|---|
| Wild-type | A:X | 0.34 ± 0.12 |
| F:Y | 0.35 ± 0.07 | |
| G:Y | ~ 0 | |
| Extended | A:X | 0.20 ± 0.03 |
| B:X | 2.0 ± 1.0 | |
| C:X | 0.27 ± 0.11 | |
| D:X | 0.44 ± 0.08 | |
| E:X | ~ 0 | |
| F:Y | 0.30 ± 0.04 | |
| G:Y | ~ 0 |
a
The observed rate of unwinding was obtained by fitting the single-exponential equation to the native gel helicase data as described in the Materials and Method section of the paper. The errors are the standard deviations from the means of at least two independent data sets.