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The effect of different reducing agents on protein resolution in 2D gels. 50 μg aliquots of total Xenopus egg extract were dissolved in RB containing 7 M urea, 2 M thiourea, 4% CHAPS, 0.5% ampholytes and different reducing agents. 2DE separation was conducted as described in Materials and Methods. (A) 20 mM DTT, (B) 2 mM TBP, (C) 10 mM TCEP.
