Figure 3.

BubR1 ABBA²⁷² and ABBA³⁴⁰ Are Essential for the SAC

(A) HeLa FRT/TO cell lines stably expressing inducible siRNA-resistant, FLAG-mRuby-tagged, wild-type or mutant BubR1 were transfected with control siRNA (siCTR) or siRNA against BubR1. Immunoblotting analysis shows the relative expression levels and the efficiency of depletion. Actin is a loading control.

(B) Mitotic timing of HeLa FRT/TO cell lines in (A) was measured with or without addition of tetracycline (tet). At least 80 cells per condition were analyzed. Results representative of three independent experiments.

(C) Experiments were performed as in (B) except that nocodazole (0.33 μM) was added at the beginning of the filming. At least 80 cells per condition were analyzed. Results representative of three independent experiments. See also Figure S2.