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. 2016 Dec 23;7:626. doi: 10.3389/fimmu.2016.00626

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Copyright © 2016 Sanmarco, Visconti, Eberhardt, Ramello, Ponce, Spitale, Vozza, Bernardi, Gea, Minguez and Aoki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxic T cells from chagasic patients are less functional than those from healthy individuals. (A) Mean fluorescence intensity of TCRζ-chain in CD3+CD4+ and CD3+CD8+ cells; (B) frequency of CD107a+ cells, IFNγ, TNF, and IL-2-producing CD8+ T lymphocytes from chagasic patients (n = 5) and seronegative donors (n = 5) after PMA/ionomycin stimulation. (C) Frequency of CD107a+ cells, IFNγ, TNF, and IL-2-producing NT+ and NT− gated in CD8+ T lymphocytes from chagasic patients (n = 4) after anti-CD3+ anti-CD28 stimulation. (D) Percentage of CD39+ and CD73+ leukocytes from seropositive (n = 20) and seronegative (n = 14) individuals (*p < 0.05 and **p < 0.01).