Figure 3.

Post-symptomatic AICAR treatment does not increased mitochondrial biogenesis in skeletal muscle. (A) Representative western blot showing steady-state levels of COXI (CIV), SDHA (CII), PGC-1α, ATPase-α (CV), UQCRC2 (CIII) and NDUFB8 (CI) in quadriceps homogenates at 7.5 months old, after post-symptomatic AICAR treatment. (B) Quantification of the protein levels showed in A, relative to β-Actin and to the vehicle-treated control group. (C) mtDNA levels in homogenates from quadriceps femoralis and gastrocnemius determined by qPCR. (D) Relative expression of transcripts coding for the contractile proteinsMHC: IIb, IIx, IIa and I in the quadriceps femoralis muscle Data (n = 5/group) are presented as mean ± SEM, and unpaired Student’s two-tailed t-test was done for pair wise comparisons (untreated versus treated).