Table 2.

RTT Murine Model phenotype comparison. Table comparing the various behavioural phenotypes examined in this RTT rat model to the earliest reported abnormal findings for each of these features in various RTT mouse models to date

	RTT rat model (current study)		Earliest reported RTT mouse models
Feature evaluated	Male	Female	Male	Female
Reduced brain weight	PND 14+	PND 21+	3 weeksa (Bird, Jaenisch); PND 21b – volume by MRI (Jaenisch)	PND 21 b – volume by MRI; (Jaenisch)
Hypoactivity/reduced locomotor activity	PND 21–28+	6 months+	PND 21c (Jaenisch); PND 24d (R168X); 3–8 Weekse,f,g (Bird)	3–8 weekse (Bird); PND 29–34h (Jaenisch)
Reduced motor coordination	PND 21–28+	9 months	PND 29d (R168X)	PND 30d (R168X)
Abnormal gait	PND 21–28+	PND 21–28+	3–8 weekse,i (Bird); 4 weeksj (Jaenisch)	3–8 weekse (Bird)
Breathing irregularities	PND 21–28+	18 months	PND 15–25k –response to hypoxia (Bird); 4+ weeksl (Bird)	3 weeksm (Bird)
Seizures	PND 21–28 (by observation only)	9 months +  (by observation only)	5+ weeksn  by observation (T158A); PND 40–70°,p – by EEG (Bird)	4+ monthsd – by observation (R168X); 8–12 monthso,p – by EEG (Bird)
Tremor	PND 21–28+	N/A	5 weeksj,q (Jaenisch)	PND 55r (R168X)
Hypotonicity/reduced muscular strength	*PND 21–28+	*9–12 months+	PND 22c (Jaenisch); PND 11–21g increased strength (Bird)	35–39 weekss (308); PND 11–21g increased strength (Bird)

^aBelichenko et al. 2008;

^bWard et al. 2008;

^cSchaevitz et al. 2012;

^dSchaevitz et al. 2013;

^eGuy et al. 2001;

^fSantos et al. 2010;

^gSantos et al. 2007;

^hNag and Berger-Sweeney, 2007;

ⁱGadalla et al. 2014;

^jStearns et al. 2007;

^kVoituron et al. 2009;

^lViemari et al. 2005;

^mJohnson et al. 2015;

ⁿGoffin et al. 2011;

^oColic et al. 2013;

^pD’Cruz et al. 2010;

^qChen et al. 2001;

^rWegener et al. 2014;

^sShahbazian et al. 2002.

^*Preliminary grip strength and wire hang data not presented, addressed in discussion section.