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. 2016 Jul 4;25(16):3432–3445. doi: 10.1093/hmg/ddw185

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© The Author 2016. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Knockdown of GCase in SH-SY5Y cells inhibits macroautophagy flux and increases α-synuclein protein levels. (A) Western blot for LC3B-II levels, under basal conditions, showed a significant increase in LC3B-II levels in GBA1 siRNA-treated cells. Both scramble and siRNA cells treated with Baf A1 increased LC3B-II levels to a similar extent (n = 4). (B) Autophagic protein p62 was significantly increased in GBA1 siRNA-treated cells compared to scramble-treated cells (n = 6). (C) Western blot and immunohistochemistry showed increase α-synuclein protein levels in GBA1 siRNA-treated SH-SY5Y expressing α-synuclein tagged with hemagglutinin (HA) (n = 5). Scale bar 25μm. All data represent mean ± SD * P < 0.05 vs. scram.