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. 2016 Jul 4;25(16):3564–3577. doi: 10.1093/hmg/ddw202

Figure 2.

Figure 2.

Nasal glands in EDA pathway deleted mice and Edar expression in adult glands. (A–L) are coronal sections. (A) Submucosal glands in the mid region of the nose of a 37-week-old FVB mouse, medial nasal glands (mng) are located in the mucosa either side of the nasal septum; and lateral nasal glands (lng) are located in the lateral walls of the nasal chambers; PAS Alcian Blue. (B) Higher magnification of the lateral nasal gland. (C) Higher magnification of the medial nasal gland and both nasal glands are comprised solely of serous cells; note that PAS-positive amorphous amyloid material (arrow) between submucosal gland acini is an incidental degenerative change in the medial nasal glands of older mice: see reference [13]. (D) Higher magnification of the lateral nasal glands shows a ventral area stains weakly with PAS. (E) Medial nasal gland and (F) lateral nasal gland in the FVB mouse do not stain for lysozyme; note that the nasal septum cartilage chondrocytes are lysozyme positive. Medial nasal glands in the rostral (G) and (H) mid nose regions in FVB and EdardlJ/dlJmice stain positively for cleaved caspase 3 (arrows), example shown in 3-week-old FVB mouse. (I-K) In situ hybridization detecting Edar mRNA in 3-week-old Edar Tg951/951. (I) Nasopharynx and (J) soft palate gives punctate signals (arrows) in the nasopharynx and soft palate submucosal glands and duct, nasopharynx and auditory tube ciliated epithelium, and squamous epithelium of the oral cavity. There are no hybridization signals in muscle (m). (K) Higher magnification of the Edar signals in auditory tube submucosal gland and epithelium (e). (L) There are no hybridization signals using the negative control probe DapB. Scale bars: 2.5 mm (A); 500 µm (B); 500 µm (C,D,E,F); 100 µm (I,J); 20 µm (K,L).