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. 2016 Dec 23;6:39653. doi: 10.1038/srep39653

Figure 2. Detection of BamHI/DNA interaction by mwPIFE.

Figure 2

(A) Scheme of DNA probes for detecting BamHI binding. Position of the BamHI restriction site is indicated. (B) Normalized PIFE values obtained with different probes incubated with BSA or BamHI. Error bars indicate standard deviations from measurements of three independent wells. P value was calculated by two-tailed t-test. (C) Effect of divalent cations on BamHI binding and cleavage. A 25 bp oligonucleotide with the restriction site 1 bp from Cy3 was used as a probe. Chart shows normalized PIFE values obtained upon addition of BamHI and after the subsequent wash. Error bars indicate standard deviations from measurements of three independent wells.