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. 2016 Dec 23;6:39573. doi: 10.1038/srep39573

Figure 3. Intestinal expressions of bile acid transporters and the detoxification enzyme CYP3A4.

Figure 3

(a) Compared to controls, the PSC and PSC + UC groups showed decreased ASBT mRNA expression in both the ascending and descending colon. (b) In PSC patients compared to controls, OSTβ protein levels were significantly increased in all three parts of gut. However, within the PSC + UC group, enhanced OSTβ protein expression was observed only in the terminal ileum. (c) PSC patients did not show altered CYP3A4 protein expression in the ileum; however, CYP3A4 protein expression was enhanced compared to controls in the ascending and sigmoid colon. Patients with PSC + UC showed unaltered CYP3A4 protein expression compared to in control tissue. mRNA expression levels were normalized relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Changes in protein levels were determined using densitometry analyses after normalization to β-actin as a loading control. Gene expression levels are presented as fold-change relative to control.