Figure 1. HtrA2 regulates T cell activation.

(A) WT and HtrA2^mnd2 phenotype mice. (B) The WT and HtrA2^mnd2 mutant mice CD4⁺ T cell populations were analyzed by flow cytometry (n = 6). (C) A T cell proliferation response assay (MLR) was conducted to measure T cell activity of LBRM transfected with HtrA2 or mock vector (n = 3). (D) CD4⁺CD44⁺CD62L⁺ and CD4⁺CD44⁺CD62L⁺ T cell populations in WT and HtrA2^mnd2 mutant mice were analyzed by confocal scanning microscopy (n = 3). (E) CD4⁺ T cell populations in spleen, lung, and liver from WT and HtrA2^mnd2 mutant mice were examined by confocal scanning microscopy (n = 3). (F) The CD4⁺ T cell populations in colon from WT and HtrA2^mnd2 mutant mice were measured by immunocytochemistry (n = 3). Data are mean ± standard deviation of three independent experiments (***P < 0.01).