Figure 3. ADPR-induced Currents in Cultured SCC-9 Cells.

(A) ADPR-induced currents at −80 mV and the I-V relationship curves for TRPM2 channels were recorded using 1-s voltage ramps of −120 mV to 80 mV which were applied every 5 s, in pH 7.3 before and after application of 20 μM ACA. Right panel: Statistical data from the triggered currents by ADPR with or without ACA (n = 18~26 cells). (B) Representative recording of ADPR-induced inward currents at −80 mV was blocked by extracellular 1 mM Zn²⁺. Right panel: Statistical data from the triggered current by ADPR with or without 1 mM Zn²⁺ (n = 18~22 cells). ***p < 0.001 compared with the currents before application of 20 μM ACA or 1 mM Zn²⁺.