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. 2016 Dec 23;6:38471. doi: 10.1038/srep38471

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Real-time PCR revealed that TRPM2 mRNA in group shRNA_TRPM2 was significantly decreased compared with that of the control and sham groups. TRPM2 mRNA levels were decreased by about 65.6% in SCC9 cells, with no significant difference of TRPM2 mRNA levels found between control and shRNA_Vec group (p > 0.05, n = 3). (B) Western blotting revealed transfection of cells with TRPM2 shRNA for 48 hours significantly suppressed endogenous TRPM2 protein expression in SCC9 cells, with no significant difference between control and shRNA_Vec group (n = 3). (C) Transwell assay showed the migrated and stained cells at 24 hours after being transfected with or without shRNA_TRPM2 plasmid. (D) Quantitative results for the transmembrane ability of each group of cells (n = 3). **p < 0.01 represents cell migration in shRNA_TRPM2 transfected group compared with the shRNA_Vec blank group at 24 h transfection.