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. 2016 Oct 26;32(1):91–103. doi: 10.1093/mutage/gew050

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© The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — SERS spectra of lipid extracts of brain tissue from male (upper panel) and female (lower panel) zebrafish post-exposure with one of four CBNs. Fish (2 males and 2 females) exposures were conducted in 10 L glass tanks. CNP exposure (0.1 mg L⁻¹) was initiated and run for 21 days. Post-exposure and isolation of brain tissue, lipids were extracted; these were then mixed with Ag NPs prior to SERS. The spectral data were then inputted into MATLAB and preprocessed following wavelet de-noising, cut to 400–1800 cm⁻¹, second differentiation and vector normalisation. —, Control;—, C₆₀ fullerene-treated; —, Long MWCNT-treated; —, Short MWCNT-treated; and, —, SWCNT-treated.