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. 2016 Dec 23;6:39424. doi: 10.1038/srep39424

Figure 6. Detection of improved emulsifier properties of PhaPAh glutamine to leucine mutants by multiple methods.

Figure 6

(A) Emulsibility of oils by various concentrations of wild type and the indicated mutants of PhaPAh. Error bars represent standard error of the mean (SEM) from 3 separate competition assays. (B) Water-oil interfacial tension in the presence of various concentrations of wild-type and the indicated mutants of PhaPAh. (C) Comparison of contact angles of drops of 25 mg/L protein solutions of wild-type PhaPAh and the indicated mutants on hydrophobic PHBHHx films. (D) Hydrophobic exposure of PhaPAh wild-type and the indicated mutants at 30 °C detected by ANS fluorescence. (E) Surface tension of solutions of PhaPAh wild-type and the indicated mutants as a function of protein concentration. Error bars represent standard error of the mean (SEM) from 3 separate measurements.