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. 2016 Dec 23;6:39318. doi: 10.1038/srep39318

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Copyright © 2016, The Author(s)

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(a) Sperm obtained from the cauda epididymis were stained by hematoxylin and eosin (H&E). Sperm from Mkrn2 knockout mice were deformed: the sperm tails bent forward in the midpiece and the heads were round and irregular (arrow). Scale bar = 50 μm. (b) The proportions of abnormal sperm were calculated from five randomly selected microscopic fields in a double-blinded manner. Data are represented as the means ± SE. of five random microscopic fields. The sperm count (c),motility (d), and velocity (e) were measured by computer-assisted sperm analysis (CASA) from six different mice of corresponding phenotypes. Data are represented as the means ± SE. of six mice. Error bars represent SE. *P < 0.05, **P < 0.01 (two-tailed Student’s t test).