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. 2016 Dec 23;6:39849. doi: 10.1038/srep39849

Figure 4. Lanf is responsible for the suppression of Otx2 in the anterior neural plate and for the induction of FoxG1 expression in this region.

Figure 4

(a–d) In the control (a), the expression of Otx2 is suppressed (arrow) in the anterior neural plate (100%, n = 35), but it is strongly activated (arrow) when Lanf is downregulated with an anti-sense MO targeting Lanf mRNA (70%, n = 50) (b) In contrast, it appears to be more broadly suppressed (arrow) if Lanf mRNA is overexpressed (76%, n = 55) (d) compared with the control EGFP mRNA overexpression (97%, n = 37). Anterior is at the top; dorsal is to the left. (e–h) An effect opposite that caused by Lanf upon Otx2 expression is observed in the case of FoxG1 expression (arrow) (100%, n = 30; 65%, n = 40; 95%, n = 42 and 70%, n = 45, respectively). Anterior is to the right; dorsal is at the top (see Materials and Methods).