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. 2016 Dec 23;6:39622. doi: 10.1038/srep39622

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative AFM topography (of 5 areas) of aggregates obtained from pH 2.5 insulin fibril dissection in the presence of Que (c_Que: c_fib 1:1). (b) Representative AFM topography (of 5 areas) of aggregates obtained from pH 2.5 insulin fibril dissection in the presence of β-carotene (c_β-carotene: c_fib 1:1). (c) Propensities of the different secondary structures in 242 (Que condition) and 102 (β-carotene condition), respectively, TERS spectra on each 3 aggregates. For comparison, the reported values for mature fibrils generated under undisturbed conditions¹⁸ and native insulin are included. Error bars indicate the maximum deviation.