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. 2016 Dec 23;6:39141. doi: 10.1038/srep39141

Figure 6. Overexpression of Babo-a rescued the miR-34-induced defective γ lobe phenotype in MB neurons.

Figure 6

Confocal images show lobe pruning phenotypes of MB neurons in flies with ectopic overexpression of Babo-a (a), ectopic overexpression of miR-34 (b), or the overexpression of both (c). FasII staining reveals the medial γ lobes (arrowheads, faint magenta staining) and dorsal α and medial β lobes (arrows, strong magenta staining). In the lower panels, GAL4-OK107-driven mCD8::GFP (green) shows the morphology of α lobes (arrows), α′ lobes (double arrows), and γ lobes (arrowheads). Overexpression of Babo-a in MB neurons had no observable effect on the MB lobe pruning phenotype (a). Ectopic miR-34 overexpression in MB neurons caused defective γ lobe pruning (double-arrowheads, b). The defective γ lobe pruning phenotype was rescued by overexpression of Babo-a in MB neurons with ectopic miR-34 overexpression (c). Fly genotypes are listed in Supplemental Table 2. Scale bar: 10 μm for all panels.