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. 2016 Dec 23;15:214. doi: 10.1186/s12934-016-0612-6

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a Comparison of three different isoprene synthases (Populus alba, Pueraria montana [Kudzu vine], and Populus trichocarpa) for isoprene production from recombinant Escherichia coli strains harboring the MVA pathway. b Effects of codon optimization and the strong RBS for expression of Populus trichocarpa IspS on isoprene production and cell growth. Culture was carried out in TB medium containing 2.0% (w/v) glycerol for 24 h at 30 °C. Open bars and closed bars represent the values obtained at 12 and 24 h of culture, respectively