Table 1.
Primers, plasmids, and Escherichia coli strains used in this study
| Names | Descriptions | References |
|---|---|---|
| Primersa | This study | |
| PaispS-F | AGACGGTCTGCCAATTATGAACC | This study |
| PaispS-R | CTCTAGATTATCTCTCAAAGGGTAGAATAG | This study |
| PtispS-F | GCC ATG GCATGTTCTGTAAGCACAG | This study |
| PtispS-R | CTCTAGATTATCTCTCAAAGGGTAGAATAG | This study |
| KispS-F | TCTCTGGAAAATGACCTTAAGG | This study |
| KispS-R | CTCTAGATTAGCAGCCGGATCCCACGTAC | This study |
| sPtispS-F | GCC ATG GCTTGCTCTGTATCCAC | This study |
| sPtispS-R | CTCTAGATTAGCGTTCGAACGGCAGAATTG | This study |
| MVA-F | GTCTAGATACCTGACGCTTTTTATCGCAAC | This study |
| MVA-R | GTCTAGAGTTTCGATAAGAGCGAACGG | This study |
| Plasmids | ||
| pTrc99A | Ptrc expression vector, pBR322 origin, lacIq, Ampr | [23] |
| pTrc99S | pTrc99A containing strong RBS | This study |
| pSTV28 | Plac expression vector, pACYC184 origin, lacZ, Cmr | Takara |
| pT-PaispS | pTrc99A containing ispS from P. alba | This study |
| pT-PtispS | pTrc99A containing ispS from P. trichocarpa | This study |
| pT-KispS | pTrc99A containing ispS from Kudzu vine | This study |
| pT-sPtispS | pTrc99A containing codon optimized ispS from P. trichocarpa | This study |
| pTS-sPtispS | pTrc99S containing codon optimized ispS from P. trichocarpa | This study |
| pTS-sPt-MVA | pTS-sPtispS containing MVA pathway from plasmid pS-NA | This study |
| pS-NA | pSTV28 containing mvaE and mvaS from E. faecalis; mvaK1, mvaK2, and mvaD from S. pneumoniae; and idi from E. Coli | [19] |
| Strains | ||
| MG1655 | E. coli K-12; F−λ−, ilvG−, rfb-50, rph-1 | ATCC 700926 |
| AceCo | MG1655 ΔackA-pta, poxB, ldhA, dld, adhE, pps, atoDA | Additional file 1: Table S1 |
| DH5α | E. coli K-12; F−, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17 (rK− mK+), phoA, supE44, λ−, thi-1 | ATCC 98040 |
| MGpPapM | MG1655 harboring pT-PaispS and pS-NA | This study |
| MGpKpM | MG1655 harboring pT-KispS and pS-NA | This study |
| MGpPtpM | MG1655 harboring pT-PtispS and pS-NA | This study |
| MGpsPtpM | MG1655 harboring pTS-sPtispS and pS-NA | This study |
| MGpsPtM | MG1655 harboring pTS-sPtispS-MVA | This study |
| ApsPtM | AceCo harboring pTS-sPtispS-MVA | This study |
aStart codons are presented in bold, and restriction sites are underlined