Fig. 5.

γ-T3- and δ-T3-induced expression of EndoR stress genes after 24 h of treatment. a Effect of γ- and δ-T3 and α-T3 on XBP-1-mRNA expression. γ-T3 (20 μg/ml) and δ-T3 (10 μg/ml) induce significant mRNA expression of XBP-1. b Effect of γ- and δ-T3 and α-T3 on chop-mRNA expression. Only γ-T3 (20 μg/ml) induces significant mRNA expression of chop. c α-T3 (5 μg/ml), δ-T3 (5 μg/ml), TRF (5–20 μg/ml), and γ-T3 (5–20 μg/ml), but not α-TOC (5 μg/ml), induce the alternative splicing of XBP-1. Details about the Pst1 digestion are provided in the “Methods” section. Tunicamycin (TM) was used as a positive control of EndoR stress induction. Asterisks indicate significant differences (p value ≤0.05) between treated cells vs control (CC)